Signalling

Part:BBa_K5371006

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


GAL4

The GAL4/UAS system is a key tool for studying gene expression and function in yeast, particularly involving galactose-induced genes. In this system, the Gal4 transcription factor serves as a positive regulator, recognizing and binding to the upstream activating sequence (UASG) present in the promoters of target genes. The GAL genes, essential for yeast growth in a galactose-rich environment, include both structural genes (such as GAL1, GAL2, GAL7, and GAL10) and regulatory genes (GAL4, GAL80, and GAL3). The activation of these genes relies on Gal4, which binds to UASG and initiates transcription, ensuring the expression of genes necessary for galactose transport and its metabolism through glycolysis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 1813
    Illegal SapI site found at 502


[edit]
Categories
//cds/transcriptionalregulator/activator
//regulation/positive
Parameters
None